Hence, we examined the degree of fading after samples underwent AC electric-field mixing. The bottom and top of each box mark the 25th and 75th percentiles, respectively. Furthermore, past studies applied AC electric field mixing to pathology specimens for the purpose of effective intraoperative diagnosis. Dynamical regulations of protein—ligand bindings at single molecular level. Results Following AC electric-field mixing, there were no clear differences in microtubule morphology between the control group and the AC electric-field mixing group Fig. Focus matches the whole and we only adjusted gain so that microtubule contrast became clear; otherwise, we did not perform any type of image editing.
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We showed the Relative Intensity RI for each concentraion of primary antibody and each frequency as a box-and-whisker plot in Fig. Discussion We showed that AC electric-field mixing increased the intensity of immunofluorescence staining of microtubules.
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In this method, an alternating-current AC electric field is used to promote the antigen—antibody reaction within the microdroplet. Box-and-whisker plots of Relative Intensity for each condition. National Center for Biotechnology InformationU. Similarly, AC electric-field mixing did not destroy the form of murine blastocytes Supplementary Fig. Figure 4 shows the shapes of the microdroplet observed at each frequency Supplementary Movies 2—7.
Transformation of microdroplets has been attempted previously using vibration, acoustic actuation, microwave irradiation, and physical methods 101112 Journal of neuroscience methods.
Freshly prepared immune complexes with intermittent microwave irradiation result in rapid and high-quality immunostaining.
There was no clear difference in a microtubule staining between frequencies. Rapid mixing of sub-microlitre drops by magnetic micro-stirring.
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Past reports focused on the reduction in staining time, and did not devote attention to reduction of cost. Promotion aikta the antigen-antibody reaction allows staining with highly diluted antibody and may reduce fading. This article has been cited by other articles in PMC.
Integrated microfluidics system using surface acoustic wave and electrowetting on dielectrics technology. Previously, this device has only been applied to histological analysis of flat structures like immunostained slices of surgery specimens.
The primary antibodies were mouse monoclonal anti—acetylated tubulin antibody 1. Egg staining Eggs were subjected to immunofluorescence staining to detect microtubules, as previously described In all cases, the mean RI was higher in the AC electric-field mixing group than in the control group.
Eggs were subjected to immunofluorescence staining to detect microtubules, as previously described Results Following AC electric-field mixing, there were no clear differences in microtubule morphology between the control group and the AC electric-field mixing group Fig.
3550k Published online Oct This technique has been applied to introduction of mRNA 19 and immunostaining of murine eggs At a dilution of 1: Mice were stimulated for egg collection according to a previous report 5 It is located on the inside of the right arm sunglasses just by the iCapture charging port. Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating-current electric-field mixing of microdroplets.
Immunofluorescence staining is a very important procedure for evaluating the functions of cells, including human and murine eggs 123. To date, several methods for effective immunostaining have been used, including microwave 1516ultrasound 17and high quality reagents Short time bleaching of fluorescein isothiocyanate.
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To evaluate the intensity 350 microtubules staining quantitatively, we used the ImageJ software version 1. Although additional studies are required, we predict that AC electric field mixing may reduce the consumption of the antibody to some extent. There were no clear macroscopic differences in chromatic degree between frequencies at this dilution. Supplier data sheets for all three antibodies recommend that they should be used for immunofluorescence staining at a dilution of 1: Taking Expression of Interest at this stage Expecting pups around June Until confirmed, were just taking peoples details and will keep everyone updated We.